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KMID : 1161520010050010071
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Animal Cells and Systems
2001 Volume.5 No. 1 p.71 ~ p.75
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Differential expression of spin transcripts: Oocyte and somatic types
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Oh Berm-Seok
Hwang Sue-Yun
Solter Davor
Knowles Barbara
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Abstract
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Spin is an abundant maternal transcript comprising up to 0.2% of the total mRNA stock in mouse oocyte, whose protein product is associated with the meiotic spindle. We have identified a new isoform of Spin transcript containing a distinct 5'?untranslated region and the N?terminus of encoded protein. Northern blot and RT?PCR analysis showed that the new isoform is expressed in embryos and most of adult tissues, while the previously identified transcript is expressed solely in mouse oocyte. We thus designated these two Spin isoforms as somatic type and oocyte type, respectively. To investigate the underlying mechanism for the differential expression, genomic structure of Spin was examined. Spin exists as multiple copies in the genome, some of which appears to be pseudogenes, and characterization of Spin genomic clones indicates that oocyte? and somatic ?isoforms were generated by alternative splicing. The complex organization of Spin genomic locus and its multifaceted control of expression provide a good model to study the molecular mechanisms of elaborate genome usage in mammals.
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KEYWORD
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Spin, Oocyte type, Somatic type, Alternative splicing, Genomic structure
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